Composite

Part:BBa_K4621153:Design

Designed by: Anyang Chen   Group: iGEM23_SCUT-China   (2023-10-05)
Revision as of 18:02, 5 October 2023 by Arml (Talk | contribs) (→‎References)

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It consists of the LPMO hypothetical promoter and EctOperon.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3437
    Illegal NotI site found at 781
    Illegal NotI site found at 1666
    Illegal NotI site found at 1984
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 963
    Illegal XhoI site found at 1137
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3437
    Illegal NgoMIV site found at 998
    Illegal NgoMIV site found at 1404
    Illegal NgoMIV site found at 1410
    Illegal NgoMIV site found at 1701
    Illegal NgoMIV site found at 2750
    Illegal NgoMIV site found at 2942
    Illegal NgoMIV site found at 3452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 586
    Illegal BsaI.rc site found at 766
    Illegal BsaI.rc site found at 1548
    Illegal BsaI.rc site found at 2076
    Illegal BsaI.rc site found at 2331
    Illegal BsaI.rc site found at 2421
    Illegal BsaI.rc site found at 2496
    Illegal SapI site found at 624


Design Notes

The homology arm and the GC content of the sequence were considered when designing the primers for each fragment.


Source

This part is derived from SCUT-3 genome.

References

[1]Garcia-Estepa R et al.Sys Appl Microbiol, 2006, 29 (8) :626-633