Composite

Part:BBa_K4621152:Design

Designed by: Anyang Chen   Group: iGEM23_SCUT-China   (2023-10-05)
Revision as of 17:58, 5 October 2023 by Arml (Talk | contribs) (→‎References)

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It consists of the chi19C hypothetical promoter and EctOperon.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3468
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3468
    Illegal NotI site found at 812
    Illegal NotI site found at 1697
    Illegal NotI site found at 2015
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
    Illegal XhoI site found at 994
    Illegal XhoI site found at 1168
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3468
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3468
    Illegal NgoMIV site found at 1029
    Illegal NgoMIV site found at 1435
    Illegal NgoMIV site found at 1441
    Illegal NgoMIV site found at 1732
    Illegal NgoMIV site found at 2781
    Illegal NgoMIV site found at 2973
    Illegal NgoMIV site found at 3483
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 617
    Illegal BsaI.rc site found at 797
    Illegal BsaI.rc site found at 1579
    Illegal BsaI.rc site found at 2107
    Illegal BsaI.rc site found at 2362
    Illegal BsaI.rc site found at 2452
    Illegal BsaI.rc site found at 2527
    Illegal SapI site found at 655


Design Notes

The homology arm and the GC content of the sequence were considered when designing the primers for each fragment.


Source

This part is derived from SCUT-3 genome.

References

[1]Garcia-Estepa R et al.Sys Appl Microbiol, 2006, 29 (8) :626-633