Composite

Part:BBa_K4621072

Designed by: Ruikang Chen   Group: iGEM23_SCUT-China   (2023-10-04)
Revision as of 16:56, 5 October 2023 by Conerrr (Talk | contribs)


Fusion promoter composed of Pro1007 and ProP

Pro1007 contains the promoter of GQS52_24880 in SCUT-3. The fusion promoter composed of Pro1007（BBa_K3880007）and ProP（BBa_K4621004）can control the gene expression switch while controlling its expression intensity. Usage, Biology and Characterization Pro1007 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1007 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 120
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 162
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None