Part:BBa_K4621094
Fusion promoter composed of Pro755 and ProP
Pro755 contains the promoter of rpsD（GQS52_23440）in SCUT-3. The promoter model predicted that the promoter expression intensity was about 75% of Pro1007（BBa_K3880007）. The fusion promoter composed of Pro755（BBa_K4621043）and ProP（BBa_K4621004）can control the gene expression switch while controlling its expression intensity. Usage, Biology and Characterization Pro755 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro755 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 168
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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