Part:BBa_K4621091
Fusion promoter composed of Pro252 and ProP
Pro252 contains the promoter of rplU（GQS52_18570）in SCUT-3. The promoter model predicted that the promoter expression intensity was about 25 % of Pro1007（BBa_K3880007）. The fusion promoter composed of Pro252（BBa_K4621040）and ProP（BBa_K4621004）can control the gene expression switch while controlling its expression intensity.
Usage, Biology and Characterization
Pro252 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro252 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 81
Illegal NgoMIV site found at 98 - 1000COMPATIBLE WITH RFC[1000]
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