Composite

Part:BBa_K4621150:Design

Designed by: Anyang Chen   Group: iGEM23_SCUT-China   (2023-10-05)
Revision as of 16:09, 5 October 2023 by Arml (Talk | contribs) (→‎References)

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It consists of the chi18A hypothetical promoter and EctOperon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3369
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3369
    Illegal NotI site found at 713
    Illegal NotI site found at 1598
    Illegal NotI site found at 1916
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 123
    Illegal XhoI site found at 895
    Illegal XhoI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3369
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3369
    Illegal NgoMIV site found at 930
    Illegal NgoMIV site found at 1336
    Illegal NgoMIV site found at 1342
    Illegal NgoMIV site found at 1633
    Illegal NgoMIV site found at 2682
    Illegal NgoMIV site found at 2874
    Illegal NgoMIV site found at 3384
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 518
    Illegal BsaI.rc site found at 698
    Illegal BsaI.rc site found at 1480
    Illegal BsaI.rc site found at 2008
    Illegal BsaI.rc site found at 2263
    Illegal BsaI.rc site found at 2353
    Illegal BsaI.rc site found at 2428
    Illegal SapI site found at 556


Design Notes

The homology arm and the GC content of the sequence were considered when designing the primers for each fragment.


Source

This part is derived from SCUT-3 genome.

References

[1]Garcia-Estepa R et al.Sys Appl Microbiol, 2006, 29 (8) :626-633