Coding

Part:BBa_K4645001:Design

Designed by: Ruixuan Zhu   Group: iGEM23_HZAU-China   (2023-10-02)
Revision as of 15:07, 5 October 2023 by Ruixuan Zhu (Talk | contribs)


NeuA: scFv ( single-chain fragment variable）that inhibits the immune response to the feline


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 315
    Illegal SpeI site found at 75
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 315
    Illegal SpeI site found at 75
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 315
    Illegal XhoI site found at 693
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 315
    Illegal SpeI site found at 75
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 315
    Illegal SpeI site found at 75
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

On the basis of the expression of functional target proteins, 6*His tag is added, which can help us better obtain high-purity target proteins. Additionally, an OmpA signaling peptide was fused to the N terminal of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization.


Source

Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies.The amino acid sequence of one of the most effective monoclonal antibodies REGN 1908 was obtained through literature review.

References