Coding

Part:BBa_K4614002:Design

Designed by: Miaodi Feng   Group: iGEM23_CAU-China   (2023-09-30)
Revision as of 14:28, 5 October 2023 by FMD (Talk | contribs) (→‎Design Notes)


Lnak-R5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 529
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 529
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 529
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 529
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used GS linker in the design of IPN and R5 fusion proteins

Source

IPN comes from the genome of Pseudomonas syringae.

References