Regulatory

Part:BBa_K4621045

Designed by: Anyang Chen   Group: iGEM23_SCUT-China   (2023-10-05)
Revision as of 13:08, 5 October 2023 by Conerrr (Talk | contribs)

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Pro1008

Pro1008 contains the promoter（BBa_K3880007） of GQS52_24880 in SCUT-3 without 5’-UTR. The fusion promoter composed of Pro1008 and chitin-inducible promoter can control the gene expression switch while controlling its expression intensity. Usage, Biology and Characterization Pro1008 was inserted into the chitin-inducible promoter ProP to form a fusion promoter. The regulation of gene expression can be achieved by placing it before the coding of the target gene. In our study, Pro1008 stably expressed the target gene under the premise of chitin induction to control the production of ectoine and hydroxyectoine. We verified its regulation effect by LB fermentation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 135
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 177
  • 1000
    COMPATIBLE WITH RFC[1000]


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