Signalling

Part:BBa_K233307:Experience

Designed by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil   Group: iGEM09_IBB_Pune   (2009-10-16)
Revision as of 09:38, 18 October 2009 by Swesrini (Talk | contribs) (→‎Applications of BBa_K233307)

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Applications of BBa_K233307

This is essentially an export tag and must be fused upstream of the gene of the protein that is to be secreted out of the cell into the medium.

The fusion must be in-frame so as to get a functional protein-of-interest. Once this is ensured the tag helps in directing the fusion protein( i.e. of the tag and the protein-of-interest)to the membrane bound Tat. The Tat (twin-arginine translocation) system delivers folded proteins across biological membranes 1 TorA is the signal sequence that is used in this pathway.

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UNIQ341b0879d8887cca-partinfo-00000000-QINU UNIQ341b0879d8887cca-partinfo-00000001-QINU