Composite

Part:BBa_K4890014

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-SDG   (2023-08-14)
Revision as of 10:40, 5 October 2023 by Htclamm (Talk | contribs)


MRE-Hsp70-Hid

This part is responsible to the expression of Hid gene driven by MRE in Drosophila. It consists of MRE sequence (BBa_K4890002), Hsp70 sequence (BBa_K4890004) and Hid gene (BBa_K4890003). Metal response element (MRE) is derived from Drosophila melanogaster. In the cell nucleus, activated MTF-1 binds to MRE. The MRE is typically located in the promoter regions associated with genes involved in the response to heavy metals. Binding of MTF-1 to MRE activates the transcription of the corresponding genes, thereby promoting heavy metal-related gene expression. Head involution defective (Hid) gene is derived from Drosophila melanogaster. Hid induces cell apoptosis in Drosophila. Hsp70 is derived from pUAST plasmid. Hsp70 is a promoter that can bind to RNA polymerase and start transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 360
    Illegal XbaI site found at 1616
    Illegal PstI site found at 222
    Illegal PstI site found at 964
    Illegal PstI site found at 1193
    Illegal PstI site found at 1337
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 360
    Illegal PstI site found at 222
    Illegal PstI site found at 964
    Illegal PstI site found at 1193
    Illegal PstI site found at 1337
    Illegal NotI site found at 378
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 360
    Illegal BglII site found at 372
    Illegal XhoI site found at 487
    Illegal XhoI site found at 1393
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 360
    Illegal XbaI site found at 1616
    Illegal PstI site found at 222
    Illegal PstI site found at 964
    Illegal PstI site found at 1193
    Illegal PstI site found at 1337
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 360
    Illegal XbaI site found at 1616
    Illegal PstI site found at 222
    Illegal PstI site found at 964
    Illegal PstI site found at 1193
    Illegal PstI site found at 1337
    Illegal NgoMIV site found at 1322
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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