Part:BBa_K4890013
UAS-Hsp70-MTF1
This part is responsible to the expression of MTF-1 gene driven by UAS in Drosophila. It consists of UAS sequence (BBa_K3281012), Hsp70 sequence (BBa_K4890004) and MTF-1 gene (BBa_K4890001). UAS and Hsp70 are derived from pUAST plasmid. Upstream activating sequence (UAS) is a cis-acting regulatory sequence. It increases the expression of a neighbouring gene when binds to GAL4. Hsp70 is a promoter that can bind to RNA polymerase and start transcription. MTF-1 gene is derived from Drosophila melanogaster. It encodes MTF-1 (metal-responsive transcription factor-1) which is a transcription factor. MTF-1 protein can be activated by heavy metals. In the cell nucleus of Drosophila cells, MTF-1 binds to MRE to recruit RNA polymerase, in turn increasing the expression of Mto/Mtn to detoxicate metals.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 382
Illegal EcoRI site found at 2296
Illegal PstI site found at 244
Illegal PstI site found at 2054
Illegal PstI site found at 2232 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 382
Illegal EcoRI site found at 2296
Illegal NheI site found at 509
Illegal PstI site found at 244
Illegal PstI site found at 2054
Illegal PstI site found at 2232 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 382
Illegal EcoRI site found at 2296
Illegal BglII site found at 527
Illegal BamHI site found at 479
Illegal BamHI site found at 1468
Illegal BamHI site found at 1864
Illegal BamHI site found at 1891 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 382
Illegal EcoRI site found at 2296
Illegal PstI site found at 244
Illegal PstI site found at 2054
Illegal PstI site found at 2232 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 382
Illegal EcoRI site found at 2296
Illegal PstI site found at 244
Illegal PstI site found at 2054
Illegal PstI site found at 2232
Illegal NgoMIV site found at 1730 - 1000COMPATIBLE WITH RFC[1000]
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