Composite

Part:BBa_K4887022

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-BioX   (2023-09-19)
Revision as of 06:45, 5 October 2023 by Htclamm (Talk | contribs)


Expression vector of IbGBSSI knockout system

This part is the expression vector used for knockout gene IbGASSI (BBa_K4887001) in sweet potato (Ipomoea batatas).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2106
    Illegal PstI site found at 3528
    Illegal PstI site found at 3732
    Illegal PstI site found at 3762
    Illegal PstI site found at 4974
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2106
    Illegal PstI site found at 3528
    Illegal PstI site found at 3732
    Illegal PstI site found at 3762
    Illegal PstI site found at 4974
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1567
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2106
    Illegal PstI site found at 3528
    Illegal PstI site found at 3732
    Illegal PstI site found at 3762
    Illegal PstI site found at 4974
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2106
    Illegal PstI site found at 3528
    Illegal PstI site found at 3732
    Illegal PstI site found at 3762
    Illegal PstI site found at 4974
    Illegal NgoMIV site found at 1157
    Illegal NgoMIV site found at 1176
    Illegal NgoMIV site found at 2394
    Illegal NgoMIV site found at 3498
    Illegal NgoMIV site found at 3571
    Illegal NgoMIV site found at 4056
    Illegal NgoMIV site found at 4965
    Illegal AgeI site found at 6940
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None