Part:BBa_K4614201
The coding sequence that can be expressed in E. coli and induce filamentation.
SulA is an endogenous cell division inhibitor present in Escherichia coli[1]. It is transcriptionally regulated by LexA and its expression is rapidly induced upon DNA damage, effectively halting cytoplasmic division. The inhibition of cell division by SulA occurs through direct interaction with the cell division protein FtsZ. FtsZ is a homologue of tubulin that forms a Z-ring at the middle of the cell, where other division proteins associate to facilitate cytoplasmic division. The disruption of FtsZ ring formation by SulA leads to the formation of elongated filaments, allowing the cells to replicate DNA but unable to undergo division[2]. In projects, SulA was efficiently induced under the T7 promoter to induce the filamentous phenotype of Escherichia coli.
[1]Schoemaker, J M et al. “Regulation of cell division in Escherichia coli: SOS induction and cellular location of the sulA protein, a key to lon-associated filamentation and death.” Journal of bacteriology vol. 158,2 (1984): 551-61. doi:10.1128/jb.158.2.551-561.1984
[2]Mukherjee, A et al. “Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli.” Proceedings of the National Academy of Sciences of the United States of America vol. 95,6 (1998): 2885-90. doi:10.1073/pnas.95.6.2885 Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 376
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