Plasmid
PrtDEF

Part:BBa_K258008:Design

Designed by: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology   Group: iGEM09_METU-Gene   (2009-10-10)
Revision as of 07:33, 18 October 2009 by Metugen (Talk | contribs) (→‎References)

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Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 210
    Illegal PstI site found at 793
    Illegal PstI site found at 1140
    Illegal PstI site found at 3055
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4344
    Illegal PstI site found at 210
    Illegal PstI site found at 793
    Illegal PstI site found at 1140
    Illegal PstI site found at 3055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1259
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 210
    Illegal PstI site found at 793
    Illegal PstI site found at 1140
    Illegal PstI site found at 3055
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 210
    Illegal PstI site found at 793
    Illegal PstI site found at 1140
    Illegal PstI site found at 3055
    Illegal NgoMIV site found at 4706
    Illegal AgeI site found at 3189
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used whole plasmid in order to excrete a fusion protein with one of two signal tags which are TliA and LARD1


Source

Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA EGF: Human Epidermal Growth Factor

References

[1]Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD) Chan Woo Chung1, Jinsun You1, Kyeongyeon Kim1, Yuseok Moon2, Hoeon Kim3 and Jung Hoon AhnPublished: 29 January 2009 Microbial Cell Factories 2009, 8:11 doi:10.1186/1475-2859-8-11 Received: 18 November 2008 Accepted: 29 January 2009 This article is available from: http://www.microbialcellfactories.com/content/8/1/11

[2]JOSE´ LUIS PALACIOS, ISABEL ZAROR, PATRICIO MARTI´NEZ, FRANCISCO URIBE, PATRICIO OPAZO, TERESA SOCI´AS, MANUEL GIDEKEL, AND ALEJANDRO VENEGAS.Subset of Hybrid Eukaryotic Proteins Is Exported by the Type I Secretion System of Erwinia chrysanthemi.JOURNAL OF BACTERIOLOGY, Feb. 2001, p. 1346–1358 Vol. 183, No. 4.American Society for Microbiology.