Composite
Part:BBa_K4882009
Designed by: Yuqi Fu Group: iGEM23_Hangzhou-SDG (2023-10-04)
Pmcl1 (short)-KillerRed-SV40 NLS-TtrpC
This is a complete suicide switch cassette consisting of a hemolymph inducible promoter Pmc1 (short), a red fluorescent protein KillerRed fused with an SV40 nuclear localization signal (NLS), and a trpC terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 489
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2300
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 45
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1737
Illegal BsaI.rc site found at 2028
[edit]
Categories
Parameters
None |