Part:BBa_K4882006
Pmcl1 (short)-MCL1ss-LqhIT2-TtrpC
This is a complete expression cassette consisting of a hemolymph inducible promoter Pmc1(short), a Mcl1 secretory signal peptide fused with an insect-specific toxin LqhIT2, and a trpC terminator.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 489
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1731
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 45
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Metarhizium anisopliae is an entomopathogenic fungus widely used as a biopesticide. However, it is less efficient than most chemical pesticides. To improve the efficiency of M. anisopliae as a fungal biopesticide, we choose to introduce the LqhIT2 toxin into the fungus. To promote the secretion of the toxin, a Mcl1ss is linked at the 5’ end of the LqhIT2 gene. We put Mcl1ss-LqhIT2 (BBa_K4882007) downstream of Pmcl1 (short, BBa_K4882000). This design limited the expression of LqhIT2 inside the insect body and improved the biosafety of our product. A trpC terminator was also included to form the complete expression cassette.
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