Regulatory

Part:BBa_K4882000

Designed by: Yuqi Fu   Group: iGEM23_Hangzhou-SDG   (2023-06-09)
Revision as of 11:48, 4 October 2023 by Yuqi Fu (Talk | contribs)


Pmcl1 (short), a truncated version of hemolymph inducible promoter from Metarhizium anisopliae

The Pmcl1 promoter is responsible for regulating the collagen-like protein MCL1 in M. anisopliae. Pmcl1 is a hemolymph inducible promoter that turns on only in insect hemolymph (Wang et al., 2006). iGEM16_NYMU-Taipei registered the full-length Pmcl1 as BBa_K2040100. Compared to the full-length Pmcl1 (2764bp), a truncated, shorter version of Pmcl1 (1586bp) can lead to a twofold increase in downstream gene expression (Kanjo et al., 2019).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 489
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 45
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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