![](https://parts.igem.org/images/partbypart/icon_terminator.png)
Terminator
Part:BBa_K4800001
Designed by: Yifei Wu Group: iGEM23_NJTech-China-B (2023-10-04)
rrnB T1 terminator
Carboxylate reductase from Mycobacterium marinum(Mutation of glutamine at position 302 to glutamate)
Fig.1 Catalytic pocket for MmCAR
MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino acid glutamine to glutamic acid through acid-base mutation. The mutated Q302E has a significant improvement compared to the original one
Result:
Fig.2 Q302E and MmCAR produce 1,5-PDO in KA30
Through fermentation experiments, we have verified that Q302E can produce more pentanediol
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |