RBS

Part:BBa_K4634001

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-09-25)
Revision as of 04:23, 4 October 2023 by NgSweeHong (Talk | contribs)


pET28a(+) backbone

efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5242
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5242
    Illegal BglII site found at 5130
    Illegal XhoI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5242
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5242
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5257
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 303
    Illegal NgoMIV site found at 3350
    Illegal NgoMIV site found at 3510
    Illegal NgoMIV site found at 5098
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2429


[edit]
Categories
Parameters
None