DNA
Part:BBa_K4897002
Designed by: Ji Zhiyuan Group: iGEM23_BS-United-China (2023-10-03)
BS DNA-322 (BS DNA 3.0) using in L-RCA for detecting P. acne
BS DNA-50 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. Binding region is the key element in reacting with P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single strand DNA meanwhile the phi29 will generate double stranded DNA through amplification primers
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 161
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 161
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 161
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 161
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |