Regulatory

Part:BBa_K206001:Design

Designed by: Amelia Hardjasa   Group: iGEM09_British_Columbia   (2009-10-17)
Revision as of 23:55, 17 October 2009 by Registry (Talk | contribs)

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pBAD weak


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No special considerations


Source

Site-directed mutagenesis on BBa_I13453 with the following primers: Forward: TAATCTTATGGATAAAAAAGCTATGGCATAGC Reverse: GCGGATCCTACCTGACGCTTTTTATC

References