Coding

Part:BBa_K4989000:Design

Designed by: Athanasia Arampatzi   Group: iGEM23_Thrace   (2023-10-02)
Revision as of 19:25, 2 October 2023 by Registry (Talk | contribs)

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Acetyl-CoA C-acetyltransferase (thiolase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 984
    Illegal BamHI site found at 849
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 132
    Illegal AgeI site found at 873
    Illegal AgeI site found at 907
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence that we designed should have a start and ending codon and a single open reading frame (ORF). Also, the sequence should be expressed in our hosts (Lactobacillus and E.coli) and thus was optimized.


Source

Our part was synthetically produced but the sequence comes from Coprococcus sp. L2-50 DSM.

References