Coding
Part:BBa_K4989000:Design
Designed by: Athanasia Arampatzi Group: iGEM23_Thrace (2023-10-02)
Acetyl-CoA C-acetyltransferase (thiolase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 984
Illegal BamHI site found at 849 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 132
Illegal AgeI site found at 873
Illegal AgeI site found at 907 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence that we designed should have a start and ending codon and a single open reading frame (ORF). Also, the sequence should be expressed in our hosts (Lactobacillus and E.coli) and thus was optimized.
Source
Our part was synthetically produced but the sequence comes from Coprococcus sp. L2-50 DSM.