Coding

Part:BBa_K4645002

Designed by: Ruixuan Zhu   Group: iGEM23_HZAU-China   (2023-10-02)
Revision as of 06:43, 2 October 2023 by Registry (Talk | contribs)

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NeuB: scFv ( single-chain fragment variable）that inhibits the immune response to the feline

Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies. Among the resulting polyclonal antibodies, a monoclonal antibody named REGN1909 was found to be the most effective in blocking the immune response to the recombinant dimeric Fel D1. Through a literature review, it was discovered that REGN1909 had both light chain and heavy chain genes. By fusing the C terminal of the light chain with the N terminal of the heavy chain using a rigid linker (GGGGS)3, an scFv was obtained. An OmpA signal peptide was fused to the N terminal of the scFv to secrete it into the periplasmic space, where it can more effectively neutralize the antigen.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 679
    Illegal XhoI site found at 276
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None