DNA

Part:BBa_K4765126

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-02)
Revision as of 06:41, 2 October 2023 by Siliang Zhan (Talk | contribs)


ribozyme connected: H. ex mtSSB + SAHS 33020 + AnAFP contributed by Fudan iGEM 2023


Usage and Biology

This composite part combines BBa_K4765016(H. ex mtSSB),BBa_K2306003(SAHS 33020)and BBa_K4765015(AnAFP) in our ribozyme-assisted polycistronic co-expression system：pRAP.

Characterization

See more in BBa_K4765016(H. ex mtSSB),BBa_K2306003(SAHS 33020)and BBa_K4765015(AnAFP)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 880
    Illegal BsaI.rc site found at 515
    Illegal BsaI.rc site found at 596
    Illegal SapI site found at 409
    Illegal SapI.rc site found at 1804


[edit]
Categories
//collections
Parameters
proteins