Part:BBa_K4765120

ribozyme test: T7 leaky expression

Introduction

This composite part is utilized to assess the cleavage efficiency of Twister P1 and stem-loop’s ability of preventing mRNA degradation. It is regulated by T7 promoter ,lac operator and T7 terminator. From its upstream to understream includes StayGold, stem-loop, Twister P1 and mScarlet. A ribozyme is proved to have cleavage ability when green and red fluorescence are emitted at the same time. We can assess the cleavage efficiency of ribozyme based on the ratio of red-green fluorescence intensity when the stem-loop is unchanged. We can also assess stem-loop’s ability of preventing mRNA degradation based on the ratio of red-green fluorescence intensity when the ribozyme is unchanged.

Usage and Biology

This composite part is an easy and effective tool to select the fit ribozyme and stem-loop.

characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 1409
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 700
  Illegal BsaI.rc site found at 720