Generator
Part:BBa_J107202
Designed by: Massimo Bellato, Lorenzo Pasotti Group: Magni Lab (2020-06-01)
Revision as of 15:07, 1 October 2023 by Tommasovar (Talk | contribs)
Constitutive dCas9 expression cassette
When expressed in medium-copy plasmid (pSB3K3), it does not give a relevant expression burden to the host (TOP10) and it provides efficient repression of medium- and high-copy target genes via specific sgRNAs, co-expressed in low-copy plasmid using the BBa_J107201 scaffold.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1401
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1401
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1160 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1401
Illegal BamHI site found at 3439
Illegal XhoI site found at 4169 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1401
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1401
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |