Generator

Part:BBa_J107202

Designed by: Massimo Bellato, Lorenzo Pasotti   Group: Magni Lab   (2020-06-01)
Revision as of 15:07, 1 October 2023 by Tommasovar (Talk | contribs)


Constitutive dCas9 expression cassette

When expressed in medium-copy plasmid (pSB3K3), it does not give a relevant expression burden to the host (TOP10) and it provides efficient repression of medium- and high-copy target genes via specific sgRNAs, co-expressed in low-copy plasmid using the <partname>BBa_J107201</partname> scaffold.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1401
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1401
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1401
    Illegal BamHI site found at 3439
    Illegal XhoI site found at 4169
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1401
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1401
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None