Coding

Part:BBa_K4765022

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-09-30)
Revision as of 14:59, 1 October 2023 by Zhenmaoye (Talk | contribs) (→‎Introduction)


mScarlet

contributed by Fudan iGEM 2023

Introduction

mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)[1].

Usage and Biology

We constructed mScarlet into the composite ribozyme measurement module: twister ribozyme (constitutive expression) and ribozyme measurement module: twister ribozyme (leakage expression) to test the effiency of twister ribozyme and stem-loop.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 512
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


=Reference

  1. ↑ Bindels, D. S., Haarbosch, L., van Weeren, L., Postma, M., Wiese, K. E., Mastop, M., Aumonier, S., Gotthard, G., Royant, A., Hink, M. A., & Gadella, T. W., Jr (2017). mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature methods, 14(1), 53–56. https://doi.org/10.1038/nmeth.4074.
[edit]
Categories
//cds/reporter
Parameters
protein