DNA

Part:BBa_K4765119

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-01)
Revision as of 13:44, 1 October 2023 by Siliang Zhan (Talk | contribs)


ribozyme test: constitutive expression

contributed by Fudan iGEM 2023

Introduction

This composite part inculdes

Usage and Biology

We designed an assessment setup to characterize the performance of pRAP. This setup incorporates Staygold, mScarlet and two adjustable-strength RBS elements. Initially, we employed the assessment setup with the same RBS to validate regular protein expression in pRAP.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 1399
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 690
    Illegal BsaI.rc site found at 710


[edit]
Categories
//collections
Parameters
None