Coding

Part:BBa_K4765011

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-08-07)
Revision as of 12:00, 1 October 2023 by Siliang Zhan (Talk | contribs)


MysB codon optimized

contributed by Fudan iGEM 2023

Introduction

MysB (O-MT), an O-methyltrans-ferase, converts Demethyl 4-deoxygadusol into 4-deoxygadusol (4-DG), completing the production of 4-DG, the basic substrate of MAAs[1].

contributed by Fudan iGEM 2023
Figure1 The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr. 1

Usage and Biology

We performed codon optimization on BBa_K4273005 (NpR5599) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysB catalyzes the second reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 532


References

  1. ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.
[edit]
Categories
//cds/enzyme
Parameters
protein