![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4604015:Design
Designed by: Hannah Swientek Group: iGEM23_Freiburg (2023-10-01)
piG_01b (tetR_bluB)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 710
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1618
Design Notes
The start codon of the bluB was changed to ATG to act as a start for translation in E. coli. Correct overhangs had to be designed to ensure high efficiency with Golden Gate.
Source
Assembled with Golden Gate cloning.