Composite

Part:BBa_K4604014:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-01)
Revision as of 09:39, 1 October 2023 by Registry (Talk | contribs)

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piG_01a falsch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1618


Design Notes

Correct overhangs had to be designed to ensure high efficiency with Golden Gate. The start codon of the bluB was changed to ATG to act as a start for translation in E. coli.


Source

Assembled with Golden Gate cloning.

References