Regulatory
Pro chi19C
Part:BBa_K4621003
Designed by: Anyang Chen Group: iGEM23_SCUT-China (2023-10-01)
A whole functional sequence in front of the chitinase Chi19C gene, including its promoter.
This part contains the spacer sequence used to target a specific DNA fragment of a homoserine dehydrogenase gene, GQS52_08040 in SCUT-3. It must be co-expressed with a sgRNA that has a 20-base pair complementary sequence to the target gene that dCas9 can effectively silence the gene of interest [1]. In our case, we used it to target the binding site of RNP to effectively repress the transcription of the target gene. As a result, the production of the target product is intricately regulated.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 93
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |