Composite

Part:BBa_K4789009:Design

Designed by: Fangyuan Duan   Group: iGEM23_YiYe-China   (2023-09-23)
Revision as of 08:41, 30 September 2023 by Sunny Duan (Talk | contribs) (→‎Design Notes)


The MAP3K7-LUC improved plasmids


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2459
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3661


Design Notes

Of the MAP3K7 minigene, we mutated the "G" in the seventh position of the "atctgAg" motif to "C."

Source

References