Composite

Part:BBa_K4789007:Design

Designed by: Fangyuan Duan   Group: iGEM23_YiYe-China   (2023-09-23)
Revision as of 08:01, 30 September 2023 by Sunny Duan (Talk | contribs) (→‎Design Notes)

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pcDNA-pre-miR-145


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 952
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
    Illegal NotI site found at 978
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 952
    Illegal BglII site found at 12
    Illegal BamHI site found at 929
    Illegal XhoI site found at 985
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
    Illegal NgoMIV site found at 1423
    Illegal NgoMIV site found at 2764
    Illegal NgoMIV site found at 3047
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 4572
    Illegal SapI site found at 3492
    Illegal SapI.rc site found at 2613
    Illegal SapI.rc site found at 2823


Design Notes

pcDNA-pre-miR-145 was applied to overexpress miR-145 in cells.Its basic backbone is pcDNA3.0, and pre-miR-145 sequence was inserted into the basic plasmid.

Source

References

[1]Yu Furong,Liu Jie,Dong Weilei et al. The diagnostic value of miR-145 and miR-205 in patients with cervical cancer.[J] .Am J Transl Res, 2021, 13: 1825-1832.

[2 ]Li Qi,Yu Xiaohong,Yang Liangliang,MiR-145 inhibits cervical cancer progression and metastasis by targeting WNT2B by Wnt/β-catenin pathway.[J] .Int J Clin Exp Pathol, 2019, 12: 3740-3751.

[3]Zhang Xian-Yu,Ma Huan,Li Jing et al. Functional implications of miR-145/RCAN3 axis in the progression of cervical cancer.[J] .Reprod Biol, 2020, 20: 140-146.

[4]Han Xiting,Wang Qian,Wang Yan et al. Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1/microRNA-202-3p/periostin axis modulates invasion and epithelial-mesenchymal transition in human cervical cancer.[J] .J Cell Physiol, 2019, 234: 14170-14180.

[5] http://www.mircode.org/