Composite

Part:BBa_K4789004:Design

Designed by: Fangyuan Duan   Group: iGEM23_YiYe-China   (2023-09-23)
Revision as of 07:48, 30 September 2023 by Sunny Duan (Talk | contribs) (→‎References)

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miR-22-sponge-pepper


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 867
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 867
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 867
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 867
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use the “Pepper ” to visualize LncRNA MALAT1 in order to reflect miRNA expression in vivo. The pepper-MALAT1 plasmid will emit green fluorescence when HBC is added. In the healthy people, there were fewer miR-22. So fewer miRNA could bind to MALAT1.More free MALAT1 will emit fluorescence through reporter “Pepper assay”.

Source

The plasmid of pCDNA3-MCS-nPepper was obtained from Naying Biotechnology Company.

References

[1] Chen Xianjun,Zhang Dasheng,Su Ni et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.[J] .Nat Biotechnol, 2019, 37: 1287-1293.