Coding

Part:BBa_K4765009

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-09-30)
Revision as of 07:43, 30 September 2023 by Siliang Zhan (Talk | contribs)

ribozyme + B0_RBS + crtE

Introduction

LCA(256AA, 30kD)，Lens Culinaris Agglutinin, is a lectin from lentils that can recognize α-linked mannose residues. It is a common lectin that can specifically bind to the LPS on the surface of S. elongatus PCC7942[1].

Usage and Biology

In our project, the surface display of LCA lectin on E.coli can mediate adhesion between E. coli and S. elongatus PCC 7942.We fused LCA with intimin, constructingK4765110 ribozyme+strong RBS+intimin-LCA fusion+stem-loop .LCA was outwardly oriented towards the medium for recognition and binding with S. elongatus PCC7942.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 794
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

  1. ↑ Arai, S., Okochi, M., Shimizu, K., Hanai, T., & Honda, H. (2016). A single cell culture system using lectin-conjugated magnetite nanoparticles and magnetic force to screen mutant cyanobacteria. Biotechnology and Bioengineering, 113(1), 112–119. https://doi.org/10.1002/bit.25707
[edit]
Categories
//cds/membrane
Parameters
protein