Part:BBa_K4885007

1

This part includes a NGCatcher sequence BBa_K4119013 and bcd gene (BBa_K4119027) derived from Clostridium tyrobutyricum (C. tyrobutyricum) L319. It codes a fusion protein of NGCatcher and butyryl-CoA dehydrogenase (bcd). bcd gene codes for butyryl-CoA dehydrogenase which converts crotonyl-CoA to butyryl-CoA in the butyrate synthesis pathway in C. tyrobutyricum. NGTag/NGCatcher peptides are variants of the SpyTag/SpyCatcher system and are reported to be useful for peptide-peptide interaction for multienzyme assembly. NGCatcher has a high density of negative charge on its surface and is a highly charged protein. It forms a covalent bond through an isopeptide bond with NGTag rapidly which is not affected by the change of environment. The fusion protein of NGCatcher and butyryl-CoA dehydrogenase can be assembled with another enzyme fused with NGTag.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 214