Part:BBa_K4814010

Mammalian Codon Optimized EYFP

We have used the codon optimized version of EYFP by Watcher, R. M. et al (1998). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814009.

The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date.

References:

Wachter, R. M., Elsliger, M. A., Kallio, K., Hanson, G. T., & Remington, S. J. (1998). Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure (London, England : 1993), 6(10), 1267–1277. https://doi.org/10.1016/s0969-2126(98)00127-0

Sequence derived from: https://www.snapgene.com/plasmids/fluorescent_protein_genes_and_plasmids/EYFP

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]