Regulatory
Ptet*

Part:BBa_K4579000

Designed by: Alexa Morton   Group: iGEM23_Austin-UTexas   (2023-09-17)
Revision as of 22:18, 28 September 2023 by Alexakmorton (Talk | contribs)


PTet* promoter + RBS

This is an inducible promoter part compatible with Golden Gate Assembly using the Bee Toolkit (BTK) syntax (Leonard et al., 2018). This part undergoes BsaI digestion to produce a linear part with overhangs characteristic of a Type 2 part in the BTK syntax. This part consists of the pTet promoter upstream of a hammerhead ribozyme (HHRz), the latter of which helps insulate the transcript from sequence-specific interference effects. The pTet promoter contains two TetO operators that can be bound by the repressor protein TetR, causing downregulation of transcription. TetR may be removed from the TetO sites when bound by the inducer molecule anhydrotetracycline (aTc), allowing for the selective induction of transcription in cells containing both pTet and the tetR gene.

File:Davies-secretion-system-plasmid.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None