Composite

Part:BBa_K4642027

Designed by: Ryan Yapa   Group: iGEM23_City-of-London-UK   (2023-09-28)
Revision as of 18:00, 28 September 2023 by Registry (Talk | contribs)

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T7 / Gen4 373-21-24 Double AND-Gate / Venus / Double Terminator / T7 / Gen4 373-21-24 Double AND-Gat

This composite part is used for testing the presence of hsa-miR-373, hsa-miR-21 and hsa-miR-24. It has a T7 promoter for expression of functional RNA, BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642021 and the anti-miRNAs BBa_K4642022 and BBa_K4642023 Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is Venus, BBa_K1976002. This fluorescence produced can be detected with a luminometer to affirm a positive test.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None