Part:BBa_K4779006

UASprm1

UASprm1, an enhancer, encompasses the region from -280bp to -101bp upstream of the prm1 start codon in Saccharomyces cerevisiae. UASprm1 comprises three adjacent consensus pheromone-responsive elements (PREs). These PREs share the same orientation but are positioned opposite to the transcription start site, with a 5-nucleotide spacing between them. Furthermore, UASprm1 incorporates multiple sequences characterized by decreased nucleosome affinity, which contributes to enhancing promoter activity. To assess the viability of UASprm1 as a pheromone-inducible element, we positioned it upstream of the robust constitutive promoter pTEF2, referred to as UASprm1-pTEF2. We evaluated the fluorescence intensity of the engineered yeast strain BY4741-pRS415-UASprm1-pTEF2-GFP-CYC1 through flow cytometry, followed by data analysis using FlowJo and GraphPad Prism software. The results demonstrated a 57.78% increase in promoter activity for UASprm1-pTEF2 when induced with 5uM pheromone. This underscores UASprm1's capacity as a pheromone-inducing element, conferring desirable pheromone responsiveness to pTEF2.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]