Coding

Part:BBa_K4593002:Design

Designed by: Zixuan Zhou   Group: iGEM23_BNDS-China   (2023-09-26)
Revision as of 01:38, 27 September 2023 by Jianfei Song (Talk | contribs) (→‎Design Notes)


ClyC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 681
    Illegal AgeI site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence does not contain a start codon and must be cloned to vectors used for protein purification (such as pET-28 in our project) before being used.

Source

Hybrid endolysin from domains of 12 natural staphylococcal endolysins.

References

Lee, Chanyoung, et al. “Development of Advanced Chimeric Endolysin to Control Multidrug-Resistant Staphylococcus Aureus through Domain Shuffling.” ACS Infectious Diseases, vol. 7, no. 8, 28 May 2021, pp. 2081–2092. https://doi.org/10.1021/acsinfecdis.0c00812.