Composite

Part:BBa_K4642011

Designed by: Ryan Yapa   Group: iGEM23_City-of-London-UK   (2023-09-25)
Revision as of 11:42, 25 September 2023 by Registry (Talk | contribs)

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T7 / Gen4 145-199a-125b Double AND-Gate / Luciferase / Double Terminator / T7 / Gen4 145-199a-125b D

This composite part is used for testing the presence of hsa-miR-145, hsa-miR-199a and hsa-miR-125b. It has a T7 promoter for expression of functional RNA BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642005 and the anti-miRNAs BBa_K4642006 and BBa_K4642007. Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is luciferase BBa_I712019. This fluorescence produced can be detected with a luminometer to affirm a positive test.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 62
    Illegal BsaI.rc site found at 106
    Illegal SapI.rc site found at 974


[edit]
Categories
Parameters
None