Composite

Part:BBa_K4814001

Designed by: CHIEN-YUEH LIU   Group: iGEM23_PuiChing-Macau   (2023-09-25)
Revision as of 11:04, 25 September 2023 by Ginaliu (Talk | contribs)


RecA(K6)-hha

RecA(K6)-hha

This is a composite part aimed to kill the bacteria after testing for DNA damage. The RecA promoter is BBa_K629001, followed by RBS (BBa_B0034), biofilm reducing protein hha (BBa_K484000), and terminator (BBa_B0015). Experiments and data shows that by using hha, after the treatment of carcinogens, the optical density of the sample decreases more significantly than the control group (E.coli top10). Detailed information of hha can be obtained from BBa_K4814000.

BBa_K629001 [ccaataattttgt] BBa_B0034 [atatacat] BBa_K4814000 [cgcggatccgg] BBa_B0015


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 383
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None