Composite
Part:BBa_K4886001
Designed by: Ruyi Shi Group: iGEM23_Nanjing-BioX (2023-07-03)
Pthl-F/Xpk(BD)
It is the part which is responsible for the expression of F/Xpk from Clostridium acetobutylicum ATCC824 with Pthl promotor. It consists of Pthl sequence (BBa_K3443002), strong ribosomal binding site (RBS) sequence (BBa_K103015), F/Xpk sequence (BBa_K4119076) and terminator sequence (BBa_K3585002). F/Xpk is a gene that encodes phosphoketolase. Phosphoketolase is an enzyme with both the Fpk and Xpk activity. It catalyzes the conversion of fructose-6-phosphate (F6P) to erythrose-4-phosphate (E4P) and acetyl-phosphate (AcP), and the conversion of xylulose-5-phosphate (X5P) to glyceraldehydes-3-phosphate (G3P) and AcP.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1986
Illegal XbaI site found at 929 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1986
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1986
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1986
Illegal XbaI site found at 929 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1986
Illegal XbaI site found at 929 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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