Part:BBa_K4586002
ZF21.16
Part Description
Zinc finger proteins are considered one of the most unique and huge collections of proteins that are variable in their biological function and structure. In our condition this zinc finger protein recognizes a specific DNA sequence thus ZF21.16 can be combined with any other protein as transcription factor to direct its activity toward the target sequences that are located near the gene of interest.
Usage
We used this part in our design to form the internal domain of our syn notch receptor (BBa_K4586022). This internal domain is released after receptor activation and guided by the ZF21.16 to the target promoter that regulates the levels of expression of our therapeutic agent as shown in figure 1.
Figure 1: This figure illustrates the design of our biological circuit expressing our therapeutic agent under the control of the VP64 transcription module that regulates the activity of the ZF21.16minCMV promoter.
Literature Characterization
The study used ZF21 antibody for detection of ZF21 by Western blot at 1 µg/mL. For immunofluorescence, start at 20 µg/mL.
(A)western blot analysis of ZF21 in 3T3 cell tissue lysate with ZF 21 antibodies in abscence of blocking peptide while (B) the same western blot analysis in presence of blocking peptide
References
Nagano, M., Hoshino, D., Koshiba, S., Shuo, T., Koshikawa, N., Tomizawa, T., Hayashi, F., Tochio, N., Harada, T., Akizawa, T., Watanabe, S., Handa, N., Shirouzu, M., Kigawa, T., Yokoyama, S., & Seiki, M. (2011). ZF21 protein, a regulator of the disassembly of focal adhesions and cancer metastasis, contains a novel noncanonical pleckstrin homology domain. The Journal of biological chemistry, 286(36), 31598–31609. https://doi.org/10.1074/jbc.M110.199430
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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