Coding
Part:BBa_K185047:Design
Designed by: Alex Jiang Group: iGEM09_SJTU-BioX-Shanghai (2009-10-16)
RelE toxin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We add a His-tag before the stop codon, then we mutated the stop codon from UAG(wild type) to UAA.
Source
Escherichia coli str. K-12 substr. DH10B, complete genome