Composite

Part:BBa_K4789006

Designed by: Fangyuan Duan   Group: iGEM23_YiYe-China   (2023-09-23)
Revision as of 10:45, 23 September 2023 by Sunny Duan (Talk | contribs)


pcDNA-pre-miR-22

MicroRNAs (miRNAs), a novelty-defined class of regulatory genes, have revolutionized principles of classical bimolecular. These RNAs regulate the expression of a gene through inhibition of translational initiation or targeting mRNAs for degradation. MiR-22 is an oncomiR that is dyregulated in several female cancers, including cervical cancer, breast cancer and ovarian cancer [1]. Our team focus on the cervical cancer development. Previous research have discovered miR-22 are significantly downregulated in cervical cancer1[2-3].A growing number of studies highlights the role of miR-22 in cervical cancer drug resistance development [4]. LncRNAs work as ‘miRNA sponges’ through binding to specific miRNAs. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), long non-coding RNA, has been reported to be upregulated in cervical cancer tissues and promote cancer cell invasion and metastasis[5]. Pre-mir-22 could bind to the sequence of MALAT1 according to the prediction database miRcode(Figure 1)[5]. This mechanism gives rise to our idea about fusing a sponge RNA based on the sequences of lncRNA with binding sites complementary to the sequence of miRNA into a plasmid contained the reporter gene. In our study, we cloned the pre-miR22 sequence into the basic part pcDNA 3.0 plasmid. Then the miR22 could overexpressed in the Hela cell.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 952
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
    Illegal NotI site found at 978
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 952
    Illegal BglII site found at 12
    Illegal BamHI site found at 929
    Illegal XhoI site found at 985
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
    Illegal NgoMIV site found at 1423
    Illegal NgoMIV site found at 2764
    Illegal NgoMIV site found at 3047
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 4572
    Illegal SapI site found at 3492
    Illegal SapI.rc site found at 2613
    Illegal SapI.rc site found at 2823


[edit]
Categories
Parameters
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